Chemistry Abstract The purpose of this experiment was to determine the rate of photosynthesis in different concentrations of a Bicarbonate solution. My Hypothesis was that if you placed the spinach disks in the highest concentration of bicarbonate then that is where photosynthesis would happen the fastest. We cut out equal disk from a plant leaf and placed half in water and the other half a bicarbonate solution. We will write a custom essay sample on Leaf and Photosynthesis or any similar topic specifically for you Do Not Waste HIRE WRITER We then used a syringe to carefully extract the bicarbonate solution, and then we removed the air out of the syringe and created a small vacuum within the syringe and later released the vacuum.
Prepare mL of 0. The bicarbonate will serve as a source of carbon dioxide for the leaf disks while they are in the solution. Pour the bicarbonate solution into a clear plastic cup to a depth of about 3 cm 4.
Fill a second cup with only water, this is to be used as the control group for this experiment 6. Prepare the materials for both cups simultaneously so that the results are not skewed 7. Add one drop of a dilute liquid soap solution to each cup using a pipette. If there are bubbles present, make sure to get rid of them before continuing the experiment.
The soap in this case is used to wet the hydrophobic surface of the leaf, allowing the solution to be drawn into the leaf and enabling the leaf disks to sink in to the fluid.
Use a hold puncher to cut 10 leaf disks for each cup. Avoid hold punching around the ribs of the leaf. Draw the gases out of the spongy mesophyll tissue and infiltrate the leaves with the sodium bicarbonate solution. This should be done as follows: Place 5 of the leaf disks into each syringe Place the plunger back in to the syringe, without crushing the disks.
Push in the plunger until only a small volume of air along with the leaf disks remain in the syringe Put a small volume around 5 cc of sodium bicarbonate with the soap solution from the prepared cup into one syringe and a small volume of water with soap into the other syringe.
Assure that the disks are in the solution, after the plunger is inverted. Also, assure that there is no air remaining. Make sure no air remains. At this point, a vacuum is to be created within the syringe to draw the air out of the tissue of the leaf disks. After this step, the experiment is really quite simple.
To create the vacuum, hold one finger over the narrow part of the syringe while drawing back the plunger.
While holding the vacuum, swirl the leaf disks to suspend them in the solution. The solution will infiltrate the air spaces in the leaf disks, which will cause them to sink.
This procedure may have to be repeated a couple of times if it does not work the first time. Pour the solution, along with the disks, from the syringe into the appropriate clear plastic cup.
Place both cups under the light source and start the timer.I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin.
Discussion Questions: 1. Discussion Questions: 1. The possible health benefits of consuming spinach include improving blood glucose control in people Please use one of the following formats to cite this article in your essay, paper or report.
Then a pencil was used to draw a line about 1 cm from the bottom of the paper. Meanwhile, the spinach leaves were being crushed in the crucible into a more liquefied state. Once the spinach leaves were done, a pipette was used to place the spinach extract along the ling drawn around the cylinder.
Photosynthesis and Chromatography of Spinach Leaves Discussion: The chromatography of the spinach leaves was taken to establish what pigments were present in the chloroplasts of the leaves - Photosynthesis and Chromatography of Spinach Leaves Essay introduction.
The solvent carried and separated the different pigments up the cellulose paper.
Aim: The aim of this experiment is to investigate the effect of sodium carbonate and light intensity on the rate of oxygen produced by plants by observing the floating of the Spinach leaf.
During the light reactions, pigments within the thylakoid membranes absorb solar energy, water is split, and oxygen is released. (Mader, ) In this study, we are interested in examining the effects of photosynthesis in spinach leaves in the presence of different light conditions.